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 ARRAY-IT

TOOLS AND INFORMATION FOR DOING cDNA ARRAYS

Protocol for the isolation of total RNA using Trizol reagent.
- (for added info see Storey lab Technical Bulletin #MB03)

Protocol for the use of human cDNA microarrays from the Ontario Cancer Institute Microarray Centre.
- picture of a human 19K cDNA array hybridized with Cy3 and Cy5 dyes.

Link to the Ontario Cancer Institute Microarray Centre to learn more about array screening. Take their online tutorial or read their frequently asked questions (FAQ).

See examples of the use of cDNA arrays by the Storey lab in papers on:

Hibernation
Eddy & Storey (2002) (CMRS): Review of array screening and heterologous probing. Figure 1 shows Atlas rat 1.2 nylon cDNA arrays and Figure 3 shows human 19K glass slide cDNA arrays.
Storey (2004a) (Exp Biol) and Storey (2005a) (Hamburg): Hibernation research and applications to human organ preservation.

Anoxia tolerance
Larade & Storey (2002) (CMRS) for anoxia tolerant marine snails: Figure 4 shows human 19K cDNA arrays screened with mRNA isolated from normoxic versus anoxic snail hepatopancreas.
Storey (2004b) (Africa) for anoxia tolerant turtles.

Freeze tolerance and cold hardiness
Storey (2004c) (Portugal) and Storey (2004d) (Alaska) for freeze responsive genes in freeze tolerant frogs.
Storey (2006) (Minneapolis) for freeze responsive genes in freeze tolerant turtle hatchlings
Storey and McMullen (2004) (Alaska) for cold responsive genes in insects

Gene hunting in hypoxia and exercise: Storey (2005b) (Lake Louise)